摘要
目的巢式PCR方法扩增恶性疟原虫DHFR基因直接进行DNA序列测序。方法在巢式PCR程序中,设置2次退火(退火45℃45 s;退火65℃1 min),产生足量的DNA模板,进行其序列测试。结果共测试2组样本,dhfr基因序列全长218样本为647 bp,217样本为692 bp,其中218样本在170、193、341位点发生变异。结论该方法省时,节约材料,可满足现场快速调查恶性疟原虫耐药基因的要求。
Objective To directly determine sequence of DHFR of Gene of Plasrnodiurn falciparurna DNA by nest PCR amplification. Methods In nest PCR amplification, we install annealing twice（the first annealing 45 ℃ 45 s, the second annealing 65 ℃, 1 min）. It amplifies more DNA to determine sequence. Results The method saves time and economizes material. Conclusion The method satisfies the demand of a fast spot survey of the resistance gene of P. falciparurn.
出处
《中国病原生物学杂志》
CSCD
2007年第6期443-444,442,共3页
Journal of Pathogen Biology
