摘要
目的：探讨肝细胞线粒体磷脂组分的增龄性改变。方法：测定了不同月龄（６、１２、１８和２４月龄）组大鼠肝细胞（ＨＣ）线粒体磷脂及其组分、丙二醛（ＭＤＡ）、维生素Ｅ（ＶＥ）和磷脂酶Ａ２（ＰＬＡ２）水平及ＶＥ对以上各指标的影响。结果：各月龄组总磷脂水平无明显改变，１８和２４月龄组磷脂酰胆碱（ＰＣ）含量明显降低，磷脂酰乙醇胺（ＰＥ）／ＰＣ值显著升高。１８和２４月龄组ＭＤＡ和ＰＬＡ２水平较６月龄组明显上升，ＶＥ含量明显下降。各ＶＥ处理组（ＶＥＧ）ＶＥ水平显著增加，１８月龄ＶＥＧＭＤＡ和ＰＬＡ２水平明显低于非ＶＧＥ（ＮＶＥＧ），２４月龄ＶＥＧＭＤＡ和ＰＬＡ２水平与ＮＶＥＧ比较差异不显著，各ＶＥＧ磷脂及其组分水平与ＮＶＥＧ比较差异不显著，但１８和２４月龄ＶＥＧＰＥ／ＰＣ较ＮＶＥＧ明显增加。结论：线粒体磷脂组分改变是ＨＣ增龄性改变的重要特征，与脂质过氧化反应密切相关，ＶＥ对老化ＨＣ线粒体过度活跃的脂质过氧化反应有一定的防治作用。
Objective: To investigate the age related changes of the composition of hepatocytic mitochondrial phospholipids and its mechanism. Methods: The levels of hepatocytic mitochondrial phospholipid(VE) and its composition, malondialdehyde (MDA), vitamin E (VE) and phospholipidase A2 (PLA2) were measured and the effects of VE pretreatment on the above mentioned indices determined in rats of various ages (6 mo, 12mo, 18mo and 24mo). Results: There was no significant difference among the total PL levels of the four groups, but the contents of phosphoatidylcholine (PC) of the 18 mo and 24 mo groups were significantly lower than that of the 6 mo group and phosphoatidylethanolamine (PE)/PC ratio was significantly increased. There were significant increase of MDA and PLA2 levels and decrease of VE level in the 18 mo and 24 mo groups and the 4 VE pretreated groups (VEGs) had evidently higher VE contents. The MDA and PLA2 contents were significantly lower in VEG of 18 mo than in non-VE pretreated groups (NVEGs). As for levels of total PL and its composition, there was no significant difference between VEGs and NVEGs. However, the PE/PC ratios of the 18 mo and 24 mo VEGs were significantly higher than those of NVEGs. Conclusion: The altered PL composition, which is closely related to oxidative damage, plays an important role in the hepatocytic mitochondrial decay in aging. VE is effective only to some degree in inhibiting the oxidative damage induced by the altered PL composition.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1997年第2期128-130,共3页
Journal of Third Military Medical University
关键词
线粒体
磷脂
增龄性
aging
hepatocytic mitochondrial phospholipid
malondialdehyde
phospholipidase A2
vitamin E
