摘要
利用大鼠缺血模型观察预防性应用钙桔抗剂尼莫地平对大鼠缺血心肌和红细胞内钙的影响发现，缺血时大鼠心肌细胞、心肌组织和红细胞内均有钙积聚，红细胞膜钙泵活性下降；缺血前30分钟给予尼莫地平可使红细胞内钙恢复至正常水平，红细胞膜钙泵活性升高；心肌细胞和心肌组织内钙虽较缺血鼠降低，但仍高于对照组。提示：（1）缺血时细胞的钙内流可能是全身性的；（2）预防性应用尼莫地平可阻滞或减轻细胞内钙超载，同时还可保护细胞膜膜泵活性。
Rats were made ischemic by injecting isoprenaline subcutaneously, myocyte and erythrocyte free calcium was measured by the Ca2+ indicator Fura-2/AM, cardiac tissue and erythrocyte total calcium was determined by using atomic absorption spectrophotometry, and the activities of Ca2+-Mg2+ ATPase and Na+--K+ ATPase of erythrocyte membrane were measured simultaneously. The results showed (1) There was calcium overload in myocyte and cardiac tissue as well as erythrocyte during ischemia ；the activities of Ca2+-Mg2+ ATPase of erythrocyte membrane were depressedl (2) In the rats treated with nimodipine before thirty minutes of ischemia, erythrocyte calcium contentrecovered to control group level, the activities of Ca2+-Mg2+ ATPase and Na+--K+ ATPase of erythrocyte membrane increased. Although myocyte and cardiac tissue calcium dropped than that of ischemic groups, it was still higher than that of control groups. It is concluded that (1) Iinflux of calcium into cells may be a systemic process during ischemia; (2)Being used prophylactically, nimodipine can prevent or relieve calcium overload, and can also protect membrane pump activities of cell membrane.
出处
《上海医学》
CAS
CSCD
北大核心
1997年第5期253-254,共2页
Shanghai Medical Journal
基金
国家自然科学基金
关键词
缺血
钙拮剂
红细胞
钙
心肌缺血
预防
Ischemia Calcium antagonist Myocyte Erythrocyte Calcium
