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PCR检测原发性肝细胞癌WWOX基因表达及意义 被引量：1

Study on expression and significance of tumor suppressor gene WWOX in hepatocellular carcinoma tissues by real-time fluorescent quantitative PCR
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摘要 目的检测染色体普通型脆性位点FRA16D(16q23.3-q24.1)上WWOX(WW domain containing oxidoreductase)基因在原发性肝细胞癌(HCC)中的表达及意义。方法用RT-PCR、荧光定量PCR方法分别对40例原发性肝细胞癌癌组织(以下简称肝癌组织)、癌旁组织中WWOX基因的Δ6-8变异情况及其mRNA表达差异进行检测。结果WWOX mRNA在肝癌组织的表达(0.318±0.0559)显著低于癌旁组织(1.0),P<0.05;而且在肝癌组织中Δ6-8变异体的出现频率为20%(8/40),显著高于癌旁组织2.5%(1/40),P<0.05。结论WWOX基因的低表达、变异可能在肝癌的发生发展中起重要作用,并有可能作为肝癌的早期诊断指标之一。 Objective To evaluate the expression and significance of tumor suppressor gene （WWOX） in hepatocellular carcinoma （HCC） tissues. Methods Variant delta 6-8 of WWOX in patients with hepatocellular carcinoma was detected by RT-PCR and the expression of WWOX mRNA was examined by fluorescent quantitative PCR （FQ-PCR）. Results The expression of WWOX mRNA in HCC patients （0. 318 ±0. 0559） was significantly lower than that in para-carcinoma tissue （P 〈0.05）. Variant delta 6-8 was found in HCC and its frequency （20%, 8/40） was significantly higher than that in para-carcinoma tissue （2.5%, 1/40, P 〈 0.05 ）. Conclusions The decreased expression of WWOX gene and its variant may play an important role in carcinogenesis and development of HCC, and may be used as a new marker for prediagnosis of HCC.
作者 陈锦萍 王效民 张忠英 游攀 李涌 黄建炜 吴绍峰 傅锦波
机构地区 福建医科大学 厦门大学医学院附属中山医院肝胆外科 厦门大学医学院附属中山医院厦门市临床检验中心 厦门市疾病预防控制中心分子生物实验室
出处 《临床检验杂志》 CAS CSCD 北大核心 2007年第5期364-366,共3页 Chinese Journal of Clinical Laboratory Science
关键词 肝细胞癌 WWOX基因 抑癌基因 脆性位点 hepatocellular carcinoma wwox gene tumor suppressor gene frigile sites
分类号 R735.7 [医药卫生—肿瘤]
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参考文献11

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同被引文献31

  • 1王甜甜,马榕,高海东,唐鲁兵,冯进波.FHITmRNA和WWOXmRNA在乳腺癌中的表达及其临床意义[J].中国普通外科杂志,2005,14(9):687-690. 被引量：6
  • 2顾军,王梅,王雅杰,李咏梅,袁杨,薛春燕.抑癌基因WWOX在肝外胆管癌中的表达[J].世界华人消化杂志,2007,15(11):1302-1305. 被引量：6
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  • 5Melino G,De Laurenzi V,Vousden K H . p73: Friend or foe in tumorigenesis[J] . Nat. gev. Cancer,2002(2):605-615.
  • 6Aqeilan RI,Palamarchuk A,Weigel RJ,et al. Physical and Functional Interactions between the Wwox Tumor Suppressor Protein and the AP-2λ Transcription Factor [ J ] . Cancer Rcs, 2004 (64) : 8256- 8261.
  • 7Aqeilan RI, Donati V, Palamarchnk A, et al. WW domain-containing proteins,WWOX and YAP,compete for interaction with ErbB-4 and modulate its transcriptional function [J] . Cancer Res,2005 (65) : 6744-6772.
  • 8Gaudio E,Palamarchuk A,Palumbo T,et al . Physical association with WWOX supresses c-Jun transcriptional activity [J] . Cancer Res, 2006, 66 (24): 11585-11589.
  • 9Iliopoulos D,Guler G,Han SY,et al . Fragile genes as biomarkers: Epigenetic control of WWOX and FHIT in lung,breast and bladder cancer[J] . Oncogene,2005,24: 1625-1633.
  • 10Kuroki T,Trapasso F,Shiraishi T,et al. Genetic alterations of tumor suppressor gene WWOX in esophageal squamous cell carcinoma[J ] . Cancer Res,2002,62 (8) :2258-2260.

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临床检验杂志
2007年 第5期

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