摘要
利用已经制备的大豆疫霉菌的抗血清，对大豆疫霉菌的纯培养和大豆病组织进行检测。结果表明：间接法（Ｉ－ＥＬＩＳＡ）和夹心法（ＤＡＳ－ＥＬＩＳＡ）都能较好地检测纯培养的大豆疫霉菌，但在检测病组织时，夹心ＥＬＩＳＡ的特异性较强。
Antiserum was obtained with mycelial protein extract of one strain of Phytophthora megasperma f.sp. glycinea. 20 different strains of p. megasperma f.sp. glycinea in pure culture and the pathogen in naturlly infected or inoculated roots had been detected by ELISA. The results showed that direct double antibody sandwish ELISA(DAS ELISA) was more sensitive and specific than indirect ELISA for the detection of P. megasperma f.sp. glycinea in inoculated soybean, P.megasperma f.sp. glycinea was detected reliably by ELISA several days before oospores found or before symptoms developed. So DAS ELISA may be useful for quarantine or detection early of soybean roots infected with P.megasperma f.sp. glycinea.
出处
《植物检疫》
北大核心
1997年第3期138-142,共5页
Plant Quarantine
关键词
抗血清
大豆
疫霉菌
检测
酶联免疫吸附
Antiserum, Phytophthora megasperma f.sp. glycinea, I ELISA, DAS ELISA, Detection
