摘要
登录GenBank下载猪圆环病毒2型(Porcine circovirus type2,PCV-2)的全基因组序列,根据其保守区域,设计1对特异性引物,采用SYBR Green Ⅰ随机结合渗入法,建立该模式的实时定量PCR检测方法,构建了检测PCV-2型的标准DNA模版,循环阈值(Ct)与标准DNA模板在1.0×102～1.0×107拷贝·μL-1浓度范围内呈良好的线性关系,相关系数为0.999。该方法用于猪圆环病毒2型的检测具有很高的特异性,其敏感性与常规PCR相比可以提高100倍,可以用于猪圆环病毒2型的快速检测。
According to the conservative regions of PCV-2 complete genome sequences published in GenBank, a pair of specific primers was designed in this study. Using SYBR Green I random combine method, a real-time quantitative PCR was developed to detect PCV-2 standard DNA template. Results showed cycle threshold and standard DNA template had a good linearity relations between 1.0×10^2～1.0×10^7 copy ·μL^-1 and the correlation coefficient was 0.999. This method is high specific in detection of PCV-2, it is 100 times more than general PCR in sensitivity .So it could be used in celerity detection of PCV-2.
出处
《黑龙江八一农垦大学学报》
2007年第4期67-71,共5页
journal of heilongjiang bayi agricultural university
基金
国家质量监督检验检疫总局课题资助项目。
