摘要
以稻瘟病菌丝体为试材,采用SDS-CTAB法提取基因组DNA,并对影响RAPD反应的因素进行研究,建立了适用于稻瘟菌的RAPD最佳反应体系。结果表明,从菌丝体中提取的DNA质量高;25μLRAPD-PCR体系中,在模板DNA2.00μL、dNTPs1.60μL、Taq酶1.25U、引物浓度1μL、退火温度34℃时,获得的特异性谱带清晰可靠,可重复性高。
Using mycelium ofMagnaporthe grisea as material, genomic DNA ofMagnaporthe grisea was extracted by SDS-CTAB method. At the same time several factors which could affecte RAPD amplification in Magnaporthe grisea genomic DNA were analyzed and a better system was promoted. Results showed that the DNA distilled from mycelium had high quality. In 251al reaction system, when annealing temperature was 34℃, DNA template was 2.00μL, Taq was 1.25U, dNTPs was 1.60μL and concentration of material was 1μL, electrophoresis bands were clear and stable.
出处
《黑龙江八一农垦大学学报》
2007年第4期27-31,共5页
journal of heilongjiang bayi agricultural university
基金
黑龙江省研究生创新科研项目(YJSCX2007-0252HLJ)。
