摘要
给大鼠胆胰管内逆行注射牛磺胆酸钠制备急性坏死性胰腺炎动物模型，采用Ｎｏｒｔｈｅｒｎ杂交及免疫组织化学方法检测了ｈｓｐ７０基因在胰腺外分泌细胞中的表达。Ｎｏｒｔｈｅｒｎ杂交分析发现，术后１ｈ即出现ｈｓｐ７０ｍＲＮＡ的高表达，然后开始下降，术后８－１６ｈ恢复至对照组水平。免疫组织化学检测发现，术后１ｈ即可见明显的ＨＳＰ７０蛋白染色，２ｈ最强，然后开始减弱，一直持续至１６ｈ，仍高于对照组水平；阳性染色位于腺泡细胞顶部并呈大颗粒状，而基底部、细胞核及腺泡腔内未见阳性信号。推测急性胰腺炎时胰腺外分泌细胞高表达ｈｓｐ７０基因，可能与其参与大量合成的胰酶的转运及抑制胰酶激活等保护作用有关。
With the acute necrotizing pancreatitis rat model induced by giving sodium taurocholate,the expression of hsp70 gene was detected in exocrine pancreas using Northern hybridization and immunohistochemistry. Nothern hybridization analysis showed that the levels of hsp70 gene expression increased and peaked at 1h after operation,and declined to the control levels at 8-16h.In immunohistochemistry detection,the staining for HSP70 protein enhanced significantly at 1h,peaked at 2h, and then decreased,persisting for 16h or longer.The location of the large particle positive signal for HSP70 was in the apical region of the acinar cells, while very few signals for HSP70 were observed in the basal region,nucleus and acinar lumen.The results suggest that the high expression of hsp70 gene in the acinar cells may be involved in the transportation of pancrease enzymes synthesized excessively and in the prevention of early enzyme activation.
出处
《中国应用生理学杂志》
CAS
CSCD
1997年第1期46-49,共4页
Chinese Journal of Applied Physiology
关键词
坏死性
胰腺炎
胰腺
外分泌细胞
HSP70基因
acute necrotizing pancreatitis
exocrine pancreas
hsp70 gene
Northern hybridization
immunohistochemistry
