摘要
利用紫外线对克雷伯杆菌进行诱变，筛选出耐高浓度甘油且1，3-丙二醇（1,3-PD）产量较高的突变株KpⅥ．实验结果表明，在距离34cm，功率30w的紫外灯垂直照射下，最佳紫外诱变时间为6min，此时，致死率为90．9％．克雷伯杆菌经过紫外诱变后，菌落明显增大，是原始菌株的2～4倍；变异菌株KpⅥ经过6代遗传稳定性考察，甘油消耗率和1，3-PD产量稳定，且保持了较高水平．在甘油质量浓度为90g·L^-1的条件下，变异菌株KpⅥ的甘油消耗率为98．3％，甘油转化率为50．4％，1，3-PD产量为44．81g·L^-1，生产能力达到0．75g·（L·h）^-1。
A mutant Klebsiella pneumoniae （KpⅥ） strain that endure high glycerol concentration and had high 1,3-PD yield was screened by ultraviolet （UV）. The optimal mutation time was 6 min when 30 W ultraviolet plumb irradiation from 30 cm distance, the lethal ratio was 90.9%. The Klebsiella pneumoniae strain was enlarged distinctly after ultraviolet mutation, 2～4 times than original strain. The mutant strain KpⅥ had showed steady glycerol consumption rate and high 1,3-PD yield via 6 generation inherit stability review. The mutant strain＇s glycerol consumption rate was 98.3%, glycerol transformation rate was 50.4% ,1,3-PD yield was 44.8 g ~ I.-1 and the through put was0.75g·（L·h）^-1 when glycerol concentration was 90 g· L^-1.
出处
《华侨大学学报（自然科学版）》
CAS
北大核心
2007年第4期399-402,共4页
Journal of Huaqiao University(Natural Science)
基金
国家自然科学基金资助项目(20276026
20446004)
福建省科技计划重点项目(2003I020)
关键词
克雷伯杆菌
1
3-丙二醇
紫外诱变
甘油
Klebsiella pneumoniae
1,3-propanediol
ultraviolet mutagenesis
glycerol
