摘要
目的:研究雄激素对雄性去势大鼠骨代谢及骨组织形态计量学的影响。方法:选用15周龄雄性SD大鼠去睾后作为动物模型,随机分为对照组11只、模型组11只和治疗组10只,28周后行血尿生化检测、骨密度及骨组织形态计量学检查。结果:与正常对照组比较,模型组大鼠的血清睾酮水平与碱性磷酸酶水平均显著降低(P<0.01),全身及股骨中点骨密度也显著下降(P<0.05),24 h尿羟脯氨酸、尿钙、尿磷与肌酐比值显著增高(P<0.01)。有关骨组织形态计量学的指标—骨小梁体积与骨小梁平均厚度均显著下降(P<0.01),骨表面面积/体积比显著增加(P<0.01)。应用十一酸睾酮替代治疗后,上述指标全面好转。结论:雄性大鼠去睾28周后形成了骨质疏松,雄激素对去睾大鼠的骨形成与骨吸收过程均有影响,并最终影响骨骼的微观结构,早期应用十一酸睾酮替代治疗,可预防这种骨质疏松的发生。
Objective：To study the effect of androgen on osteoporosis （Op） in male rats. Methods： Thirty-two 15-week-old male Spague-Dawley rats underwent orchidectomy and then were randomly divided into 3 groups： a normal controls, a model and a andriol treated group. Biochemical markers, bone density（BMD） and bone histomorphometry were investigated after 28 weeks. Results： Orchidectomy caused a significantly decreased in the level of testosterone （ P 〈 0.01 ） , and the biochemical markers of bone formation-AKP （P 〈 0.01 ）but an increased in the biochemical markers of bone resorption-HOP/Cr and Ca/Cr in the models （P 〈0.01 ）. The whole body BMD and the cortical BMD in femur were significantly lower in the models than those in the normal controls （ P 〈 0.05 ）. The result of bone histomorphometry showed that the trabecular bone volume and mean trabecular plate thinckness were significantly decreased and S/V was significantly increased in the models than those in the normal control. After andriol treatment, all the above indexes returned to normal. Conclusion： Castrated rats could be used as an ideal animal model of male osteoporosis. The metabolism of this model presents a low bone formation and a high bone resorption, which could be prevented by early treatment with andriol.
出处
《医学研究生学报》
CAS
2007年第9期923-926,共4页
Journal of Medical Postgraduates
基金
南京市卫生局基金资助项目(批准号:J960101)
