摘要
脂肪酶是一种广泛应用的水解酶类。脂肪酶的表面展示技术不仅是脂肪酶蛋白质工程中一种有效的高通量筛选方法,而且展示的脂肪酶与自由酶相比具备更高的温度稳定性、有机溶剂稳定性等优点,其作为全细胞催化剂与传统的固定化脂肪酶相比也具备诸多优点。脂肪酶表面展示的宿主包括噬菌体、细菌以及酵母等,将分别介绍这三种宿主中脂肪酶表面展示的概况及其作为高通量筛选和全细胞等方面的应用。
Lipase is a kind of widely used hydrolase. Surface display is an efficient method of highthroughput screening for protein engineering of lipase. Besides, lipase displaying on surface of microorganism has many advantages, such as higher stability against high temperature and organic solvent, compared with free lipase, so the host strain displaying lipase can be used as whole-cell biocatalyst, which has some advantages compared with traditional immobilization of lipase. There are three kinds of host strain for displaying lipase ： phage, bacteria and yeast. The surface display of lipase in the three display systems were systematically discribed, and their current uses and possible trends in the future were discussed also.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2007年第9期97-102,共6页
China Biotechnology
基金
国家"863"计划资助项目(2003AA214061)
