摘要
酵母单杂交系统是由酵母双杂交系统衍生来的研究DNA与蛋白质之间相互作用的新型系统。系统阐述了单杂交系统的基本原理和技术路线,详细综述了其在植物抗渗透胁迫转录因子各个研究领域的应用进展,即克隆抗渗透胁迫类转录因子基因,确定已知DNA蛋-白质的相互作用,定位已证实的具有相互作用的DNA结合结构域以及验证转录激活作用。分析了当前该系统在植物抗渗透胁迫转录因子研究中存在的问题,进而对解决问题的途径进行了探讨。
The yeast one hybrid system is the new type system deriving from the yeast two hybrid system to study the interaction between DNA and protein. The principle and the technical line of this system was briefly introduced, and the progress of this system on the research of plant transcription factor gene resistant to osmotic stress was particularly summarized, such as cloning the transcription factor genes of osmosis resistance type; confirming the interaction between known DNA and protein;making sure the localization of the confirmed DNA binding domain with the interaction;also validating the transcription activation of the transcription factor gene. Furthermore,the problems existing in this system on the research of plant transcription factor gene resistant to osmotic stress was analyzed, and the develooment orosoect according to the re.search was forecasted.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2007年第9期91-96,共6页
China Biotechnology
基金
国家自然科学基金资助项目(30570990)
黑龙江省科技厅重点攻关资助项目(GB05B104)
