摘要
本研究用计算机控制、可程序升温的毛细管柱气相色谱（ＧＣ）仪分析４８株铜绿假单胞菌标准株和临床株、部分常见假单胞菌、肠杆菌的细胞脂肪酸。结果表明：月桂烯酸（Ｃ１２：１）、月桂酸Ｃ１２：０）、十三碳烯酸（Ｃ１３：１）、十三碳酸（Ｃ１３：０）、肉豆蔻脑酸（Ｃ１４：１）、十七碳稀酸（Ｃ１７：１）、十七碳酸（Ｃ１７：０）、硬脂酸（Ｃ１８：０）和花生四烯酸（Ｃ２０：４）是铜绿假单胞菌有鉴别意义的脂肪酸，组成与含量有属和种的特异性。ＧＣ法与常规细菌学方法不同，具有操作简单、省时、干扰因素少、灵敏度、重复率及自动化程度高的特点，经进一步完善和推广应用，有可能取代传统的、以形态学和生化反应为主的鉴定方法，成为病原菌检测的主要手段。
strains of Ps. aeruginosa, some strains of Pseudomonas sp. , Enterobacteriaceae selected from clinical isolates and reference strains were examined for their cellular fatty acid(CFA) composition liberated from whole cells by base hydrolysis, methylated, and analyzed by the fused-silica capillary column gas chromatography (GC) equipped with computer interface and flame ionization detector. The distinctive patterns of major fatty acids of Ps. aeruginosa include lauroleic acid (C12:1), lauric acid (C12:0), tridecenoic aced(C13:1), tridecanoic acid (C13:0), myristoleic acid (C14:1), heptadecenoic acid (C17:1), heptadecanoec acid (C17:0), stearic acid (C18:0) and arachidonic acid (C20:4). In this study, analysis of the CFAs has proven useful for species characterization and identification . In comparing with conventional identification systim, GC is an accurate, simple, efficient and relatively rapid method for the identification of Ps. aeruginosa and other Pseudomonas sp it will provides an alternative to conventional methods.
出处
《中华医院感染学杂志》
CAS
CSCD
1997年第1期7-10,共4页
Chinese Journal of Nosocomiology
