摘要
目的:探讨大鼠腹部异位心脏移植模型的外科技巧并改良此模型的制作方法,使其更加简便且成功率高.方法:对传统Ono术式的受体准备、供心切取和吻合方法等环节进行改进,采用此改良方法建立SD大鼠的腹部异位心脏移植模型.结果:共实施100例大鼠腹部异位心脏移植术,89例成功.供心缺血时间(32±5)min,整个手术耗时(60±10)min.结论:经典的Ono术式经改进后,术中缩短了供心缺血时间,降低了吻合难度,手术成功率高.整个过程均可单人操作,无需显微镜,使其易于掌握与开展.
AIM： To explore the surgical skills and modify the procedure in making animal models of heterotopic heart transplantation in rats, in order to make it more convenient and improve the success rate. METHODS： Heterotopic heart transplantations of male SD rats were performed by using Ono＇s method with modifications of preparation of recipient, donor cardiectomy and vascular anastomosis. RESULTS： Of the 100 cases receiving heterotopic abdominal heart transplantation, complete success was achieved in 89 cases. Total ischemia time was （32 ± 5 ）min, and full time of operation was （60 ± 10 ）min. CONCLUSION： This improved Ono＇s method can shorten the ischemia time, make the anastomosis easier and increase the success rate of heterotopic heart transplantation. The whole process can be completed by a single man without microscope assistance, so this method is easy to learn and perform.
出处
《第四军医大学学报》
北大核心
2007年第18期1646-1648,共3页
Journal of the Fourth Military Medical University
关键词
心脏移植
大鼠
疾病模型
动物
heart transplantation
rats
disease models, animal
