摘要
从黄瓜幼苗生长、叶片丙二醛(MDA)含量和质膜相对透性3个方面的变化证实100μmol·L-1的一氧化氮(nitric oxide,NO)供体硝普钠(sodium nitroprusside,SNP)对100mmol·L-1NaCl胁迫下黄瓜(Cucumis sativusL.)叶片氧化损伤有明显的缓解效应.NO处理后,盐胁迫下的黄瓜地上部和地下部干重显著高于未加NO的处理,叶片质膜相对透性显著低于未加NO的处理.NO能够明显诱导盐胁迫下黄瓜叶片超氧化物歧化酶(SOD)、愈创木酚过氧化物酶(GPX)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活力的上升,延缓MDA和H2O2积累,同时促进渗透调节物质脯氨酸含量的上升.说明NO提高了黄瓜叶片的抗氧化能力,减轻了盐胁迫诱导的膜脂过氧化损伤.
Effects of nitric oxide（NO）on the growth of seedlings and membrane lipid preoxidation in cucumber leaves with NO donor sodium nitroprusside（SNP）treatment in the presence of 100 mmol·L^-1 NaCl were studied.The results showed that 100 μmol·L^-1 SNP promoted the growth of cucumber seedlings and increased soluble protein content of leaves under salt stress;as well as opposing effects in electrolyte leakage and osmolity of the cucumber leaves under salt condition.Measurement of reactive oxygen species（ROS）metabolism showed that there was,compared with control,a decrease in malondialdehyde（MDA）and H2O2 content,but a significant increase in superoxide dismutase（SOD）,guaiacol peroxidase（GPX）,catalyse（CAT）and ascorbic peroxidase（APX）activities after SNP treatment.The level of proline was increased by SNP treatment under salt stress.It is suggested that SNP treatment enhanced the levels of the antioxidative activity of cucumber leaves under salt stress,declined the damage of membrane by lipid preoxidation.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2007年第5期533-538,共6页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家自然科学基金资助项目(30471183).
关键词
黄瓜
盐胁迫
一氧化氮
膜脂过氧化
cucumber
salt stress
nitric oxide
membrane lipid peroxidation
