摘要
建立了用高效液相色谱法测定AMPS含量和纯度的方法。样品用Zorbax SAX阴离子交换柱为分离柱，0．1mol／L KH2PO4溶液为流动相，检测波长为220nm进行分析。AMPS在进样量0．5～50μg范围内峰面积线性关系良好，回收率为96．0％～103．2％，方法的平均RSD为0．49％（n=6）。该方法可作为常规分析方法。
The quantitative determination of AMPS （2-Acrylamido-2-Methylpmpane Sulfonic Acid） samples was conducted by using the HPLC-DAD methods. A Zorbax SAX column （4.6 mm × 250 mmi. d., 5 tan） and a mobile phase consisting of 0.1 M KH2 PO4 solution were employed with the detection wavelength at 220 nm. The linear relationship was in the range of 0.5 - 50μg and the recoveries were between 96.0% and 103.2% with the relative standard deviation of 0.49% （ n = 6）. The HPLC-DAD method is simple and quick. Therefore it can be used as a routine analytical method for the determination of AMPS.
出处
《分析试验室》
CAS
CSCD
北大核心
2007年第9期60-62,共3页
Chinese Journal of Analysis Laboratory
基金
江苏省科技厅项目"AMPS质量检测的分析方法和标准研究"(2006191TS90061)项目资助
