摘要
目的:探讨血管内皮生长因子-C(VEGF-C)对肝门部胆管癌细胞侵袭和增殖的影响。方法:采用MTT法测定VEGF-C对肝门部胆管癌细胞系(FRH0201)细胞增殖的影响,流式细胞仪观察VEGF-C抗凋亡作用,应用3H-TdR掺入试验测定VEGF-C对FRH0201细胞同质黏附作用以及Boyden小室观察VEGF-C诱导肝门部胆管癌细胞转移的作用。结果:MTT法示VEGF-C能显著提高FRH0201的增殖活性,其效应随VEGF-C剂量以及时间增加而增加,并显著抑制细胞凋亡。在Boyden小室培养的FRH0201细胞中分别加入1、5和10ng/ml的VEGF-C刺激培养后,Boyden小室碳酸酯滤膜下层浸润的胆管癌细胞数均高于对照组。分别用上述浓度的VEGF-C诱导FRH020160、90和120min后,FRH0201细胞的黏附能力显著低于对照组。结论:VEGF-C可以促进胆管癌细胞增殖,抑制其凋亡,增强细胞侵袭能力,并且与降低细胞的同质黏附作用密切相关。
Objective： To investigate the effect of vascular endothelial growth factor-C （VEGF-C） on invasive capability and proliferation of human cholangiocarcinoma cells.Methods： The effect of VEGF-C on FRH0201 was assayed by MTT. The cycle pattern and apoptosis was assayed using flow cytometry. The effect of VEGF-C on homotypic adhesion and metastasis in FRH0201 was examined with ^3H-TdR infiltration and Boyden chamber. Results： VEGF-C could enhance the proliferation of FRH0201 in a dose and time dependent manner. And VEGF-C could inhibit cell apoptosis significantly. After cultured for 2 hours with 1, 5, 10ng/ml of VEGF-C, there were more cells in the lower chamber than the control group. After 60, 90, 120 minutes induction by 1ng/ml,5ng/ml, 10ng/ml, the cells showed significantly lower homotypic adhesion than that of the control group. Conclusions： VEGF-C could enhance the proliferation of FRH0201 and inhibit cell apoptosis. VEGF-C could decrease homotypic adhesion of FRH0201 and might be a cause of the metastasis.
出处
《中国现代普通外科进展》
CAS
2007年第4期287-290,共4页
Chinese Journal of Current Advances in General Surgery
关键词
血管内皮生长因子C
胆管肿瘤
细胞增殖
肿瘤转移
细胞黏附
Vascular endothelial growth factor C
Bile duct neoplasmsCell Proliferation
Neoplasm metastasis
Cell adhesion
