摘要
目的探讨染色体着丝粒点（Ｃｄ）的变异与ＨｅＬａ瘤细胞染色体非整倍性畸变的关系。方法用Ｃｄ分析技术研究ＨｅＬａ细胞染色体Ｃｄ的变异。结果ＨｅＬａ细胞染色体Ｃｄ缺失率为２．１０％、Ｃｄ迟滞复制率为０．２３％；与正常人胚胎绒毛细胞染色体Ｃｄ相比较，ＨｅＬａ细胞Ｃｄ缺失显著升高，而Ｃｄ迟滞复制没有显著性差异。结论ＨｅＬａ细胞染色体非整倍性畸变可能主要源于Ｃｄ缺失，而Ｃｄ迟滞复制则为非主要的。
Objective:Centromeric dots(Cd) variation of HeLa cells and its relation with aneuploidy were studied. Methods:A Cd banding technique modified by this laboratory was used Results:Frequency of Cd loss in HeLa cells was significantly higher than that of normal embryonic villi. Frequency of Cd duplication laggard showed no difference between HeLa cells and normal embryonic villi. Conclusions:Cd loss might be related with aneuploidy of the cancer cells. Cd of endoduplication chromosomes was observed.
出处
《肿瘤》
CAS
CSCD
北大核心
1997年第3期151-153,共3页
Tumor
