摘要
将PSG-CMV和PSG5-PML分别用EcoRI和BglII双酶切,构建PSG-PML载体.将质粒PSG-PML与含有型腺病毒右臂的质粒pPE3共转染至293细胞,产生含PML的重组腺病毒(Ad-PML).酶切、PCR、测序鉴定结果表明成功构建了PML的重组腺病毒,滴度达到5×107PFU/ml,并检测到PML蛋白的表达.
PSG-CMV and PSG5-PML were digested with EcoRI and we Bgl II,then transformed into DH5α to attain the recombined vector PSG-PML.Recombined plasmid PSG-PML and plasmid pPE3 with adenovirus right arm were co-transfected into cell 293,then we harvested the PML-bearing recombinant adenoviruses.The results of analysis of PCR and of restriction enzyme digestion were completely identical.The PML protein expression has been assayed successfully with the titers of 5×10^7PFU/ml.
出处
《内蒙古民族大学学报（自然科学版）》
2007年第4期433-435,共3页
Journal of Inner Mongolia Minzu University：Natural Sciences
