摘要
为测定真鲷(Pagrus major)胚胎CYP1A活性(ethoxyresorufin-o-deethylase,EROD)对于BaP暴露的响应,将受精两天后的真鲷胚胎暴露于不同浓度的BaP(0.5,0.8,1.2,1.8μg/L),采用一种对胚胎个体无损伤的方法测定胚胎EROD活性.结果表明真鲷胚胎EROD活性在BaP浓度为0.8μg/L时已经开始被诱导,活性最大值出现在最高浓度组1.8μg/L最高浓度组EROD活性值是对照组的4倍.从EROD活性对不同浓度BaP暴露的响应来看,真鲷胚胎EROD活性与BaP浓度有着明显的剂量效应关系,随着BaP浓度的升高EROD活性呈正相关上升,证实胚胎EROD活性可以做为BaP暴露的敏感生物标志物.
A non-destructive method was used to detect CYP1A activity of Pagrus major embryos exposed to different concentrations of BaP （0.5,0.8,1.2,1.8 μg/L）. In pro EROD activity was measured at 9 days post fertilization. The result showed that CYP1A activity of the embryos was inducible by BaP concentrations as low as 0.8 μg/L,with maximal induction occurring at the 1.8 μg/L BaP concentration. In pro EROD was dose-dependent with BaP exposure, and it has proved that CYP1A activity can be served as an sensitive biomarker of the BaP.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第A01期120-123,共4页
Journal of Xiamen University：Natural Science
基金
国家自然科学基金(40476048)
厦门大学校级优秀人才计划资助
