摘要
毛头鬼伞(Coprinus comatus)子实体经热水浸提、乙醇沉淀、脱蛋白和真空干燥后得到毛头鬼伞多糖。经定性化学反应和光谱鉴定,毛头鬼伞多糖不含蛋白质、核酸、酚类物质和糖醛酸,为非淀粉类中性多糖,均分子量为947 kD,完全酸水解后经气相色谱分析确定其糖基组成及其摩尔组成比为葡萄糖∶甘露糖∶半乳糖=10.5∶1.7∶1。通过对脱氧核糖体系产生的羟基自由基(.OH)的清除作用和邻苯三酚自氧化系统产生的超氧阴离子自由基(O2-.)的清除作用,对毛头鬼伞多糖体外抗氧化活性进行的研究结果表明:该多糖具有一定的抗氧化活性。
The polysaccharide was extracted from Coprinus comatus, treated by hot-water-soakage, ethanol-precipitati- on, deproteinixation,and vacuum drying. It was a non-starch polysaccharide without any protein, nuclear acid,polyphenol and uronic acid. Its average molecular weight was 947 kD. The unit of the monosaccharide was identified by gas chromatography as glucose, mannose,galactose with a mole ratio of 10.5 ： 1.7 ： 1 respectively after complete hydrolysis by acid. The antioxidant activities were investigated in different assays in vitro, namely scavenging activities of hydroxyl free radicals （ · OH） generated by Deoxyribose reaction system and superoxide anion（ O2^-· ） generated by pyrogallol autoxidation system. Results show that polysaccharide possess antioxidant capacity in vitro.
出处
《激光生物学报》
CAS
CSCD
2007年第4期438-442,共5页
Acta Laser Biology Sinica
基金
福建省教育厅专项科学基金项目(K04037)
福建省植物病毒学重点实验室建设补助费(K03005)
关键词
毛头鬼伞
多糖
理化性质
抗氧化活性
自由基
Coprinus comatus
polysaccharide
physico-chemical characteristics
antioxidant activity
free radical
