摘要
研究了异养无光照条件下不同碳源、氮源和碳氮质量比对小球藻生长及叶黄素产生的影响.结果表明,葡萄糖和硝酸钾分别是支持小球藻USTB01持续快速生长的最佳碳源和氮源.以葡萄糖和硝酸钾分别作为唯一碳源和氮源时,在初始氮质量浓度都为0.28g.L-1情况下,碳氮质量比为25∶1是促进小球藻生长的优化控制条件.硝酸钾是促进小球藻USTB01叶黄素生物合成的最佳氮源,但在碳氮质量比从15到30的范围内,小球藻细胞中叶黄素含量随碳氮质量比的升高而降低.
The effects of carbon and nitrogen sources as well as carbon-to-nitrogen mass ratio on the growth of Chlorella sp USTB01 and the production of lutein were investigated under the condition of heterotrophic culture in .dark. The results show that glucose and potassium nitrate were the best carbon and nitrogen sources to support the rapid and continuous growth of Chlorella sp USTB01 respectively. When glucose and potassium nitrate were used as the sole carbon and nitrogen sources and the initial concentration of nitrogen was 0.28 g·L^- 1, the growth of Chlorella sp increased with the increase of carbon-to-nitrogen mass ratio in the range from 15 to 25. Potassium nitrate was the best nitrogen source to promote biosynthesis of lutein by Chlorella sp USTB01 and the content of lutein in the cells of Chlorella sp USTB01 declined with the increase of carbon-nitrogen mass ratio from 15 to 30.
出处
《北京科技大学学报》
EI
CAS
CSCD
北大核心
2007年第8期766-770,共5页
Journal of University of Science and Technology Beijing
基金
国家自然科学基金资助项目(No20377047)
北京科技大学大学生创新基金资助项目
关键词
小球藻
异养培养
叶黄素
碳源
氮源
碳氮比
Chlorella sp
heterotrophic culture
lutein
carbon source
nitrogen source
carbon-to-nitrogen ratio
