摘要
目的:建立一种灵敏、快速检测沙门菌的实时荧光PCR方法。方法:针对沙门菌特异性基因invA设计引物、探针,在实时荧光PCR仪上进行扩增、检测和结果分析。结果:应用该实时荧光PCR方法,30株沙门菌检测结果均为阳性,19株非沙门菌结果均为阴性,96个肛门拭子样本增菌前后的PCR结果一致;检测灵敏度达到4 cfu/test,定量结果与培养计数无显著差异。结论:该方法检测沙门菌具有灵敏度高、快速方便的特点,且可用于定量检测。
Objective：To develop a specific and sensitive real -time PCR for the detection of Salmonella. Methods：Lysis of bacterial pellets from the samples were analyzed by 5 ＊ nuclease （TaqMan） PCR technique on a fluorescence real -time PCR instrument. Results：The PCR test correctly identified all the 30 Salmonella strains. No false negative was detected in 19 non -Salmonella strains. The sensitivity could be 5× 10^2 cfu/ml （4 cfu per reaction）. The post - enrichment identification could be completed in 2 hours. Quantitative results of PCR and culture were comparable. Conclusion：The real -time TaqMan assay can be a sensitive and simple alternative method for identification of Salmonella, potentially for quantitative analysis.
出处
《中国卫生检验杂志》
CAS
2007年第8期1468-1469,共2页
Chinese Journal of Health Laboratory Technology
