摘要
目的探讨大肠杆菌(E.coli)感染与人巨噬细胞系U937细胞凋亡的关系及核转录因子(nuclear factorkappa B,NF-κB)表达的变化。方法以Annexin V FITC/PI双染流式细胞仪检测及Hoechst 33258荧光染色观察为指标,研究E.coli感染对人巨噬细胞系U937细胞凋亡的诱导作用;用Western blot方法检测NF-ΚB的表达。结果Ho-echst 33258荧光染色结果表明当细胞与细菌浓度比较低时(1:10)可引起部分细胞凋亡,Annexin V FITC/PI双染流式细胞仪结果表明,当细胞与细菌浓度比为1:20,1:50及1:100时,细胞凋亡率与对照组相比明显增高,有显著性差异(P<0.001)。NF-κB的表达随着E.coli浓度的增加而逐渐降低。结论E.coli以剂量依赖的方式诱导U937细胞凋亡,在此过程中NF-κB的表达逐渐降低。
Objective To study the relationship of E. coli to human monocytic U937 cell apoptosis and expression of nuclear factor kappa B （NF-κB）. Methods The U937 apoptosis induced by E. coli was detected with Annexin V FITC/PI assay and Hoechst 33258 fluorochrome staining. Western blot was used to detect the expression of NF-κB. Results Annexin V FITC/PI assay and Hoechst 33258 fluorochrome staining found the apoptotic cells at multiplicity of infections （MOI） of 10：1 after E. coli had infected U937, and the percentage of apoptotic cells increased with the increase in the concentration of E. coll. The infection groups at MOI of 20： 1, 50：1 and 100：1 induced significantly more U937 apoptosis than in the control group （P〈0. 001）. The expression of NF-κB was reduced. Conclusion E. coli can induce apoptosis in human U937 cells with a down-regulated expression of NF-κB, and this might be related to the induction of apoptosis of the infected cells.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2007年第3期288-292,共5页
Chinese Journal of Histochemistry and Cytochemistry
