摘要
目的:探讨葶苈子对压力负荷性大鼠心室重构、心肌Ⅰ、Ⅲ型胶原及神经内分泌因子的影响。方法:腹主动脉不完全结扎法(abdominal aortic banding,AAB)制作大鼠心肌肥厚、心室重构模型。30 d药物干预后,测量大鼠收缩压(systolic blood pressure,SBP)、舒张压(diastolic blood pressure,DBP);称重法测定心脏指数(HW/BW、LVW/BW);放免法测定心肌血管紧张素Ⅱ(AngⅡ)、内皮素(endothelin,ET-1),血清醛固酮(aldosterone,ALD)的水平;免疫组织化学染色法观察心肌Ⅰ、Ⅲ型胶原的改变;心肌样本碱水法测定羟脯氨酸(Hydroxyproline,Hyp)的含量。结果:模型组SBP、DSP,HW/BW、LVW/BW,AngⅡ、ET-1,ALD,Hyp及Ⅰ、Ⅲ型胶原含量升高(P<0.05),免疫组化染色后观察心肌Ⅰ、Ⅲ型胶原阳性表达明显增加。葶苈子水提物改善心肌肥厚指数,降低AngⅡ、ET-1,ALD及Hyp和Ⅰ、Ⅲ型胶原含量作用显著(P<0.05)。结论:葶苈子水提物通过抑制压力负荷性心室重构大鼠RAAS和交感神经系统活性,减少神经内分泌因子AngⅡ,ET,ALD生成而抑制心肌肥大、心室重构,这可能是葶苈子直接保护心肌,抑制心肌细胞纤维化的作用机制之一。
Objective：To approach the influnce of Tinglizi on some neuroendocrine factors and type I and Ⅲ collagen in ventricular remodeling induced by Abdominal Aortic Banding （AAB） in rats. Methods ： Myocardial hypertrophy, ventricular remodeling model was induced by abdominal aortic banding （AAB） in rats. After 30 day treatment, the systolic blood pressure （SBP） 、 diastolic blood pressure （DBP） were measured; Then the ratios of LVW/BW （left ventricle weight/body weight）, HW/BW （heart weight/body weight） were calculated; The Angiotensin lI （Ang lI ） and Endothelin （ET-1） content in heart tissue and the Aldosterone （ALD） concentration of blood plasma were determined by radiolmmunoassay. The content of type I and Ⅲ collagen in myocardium were determined using immunohistochemical analysis . Hydroxy proline content in heart tissue was measured by hydrolysis method. Results： The experimental data demonstrated that Tinglizi could decrease SBP, DBP and the cardiac indexes of LVW/BW and HW/BW, significantly reduce the content of ANg Ⅱ , ALD and ET, decrease the total collagen content and type I and Ⅲ collagen （P〈0. 05）.Conclusion：Tiglizi can significantly attenuate the experimental ventricular remodeling; the mechanism is related with its ability to inhibit the activation of rennin-angiontensin-aldosterone system （RAAS） and systema nervosum sympatheticum （SNS）, decrease the content of neuroendocrine factors （Ang II , ET, ALD）.
出处
《中药材》
CAS
CSCD
北大核心
2007年第8期963-967,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金资助项目(30572379)
国家中医药管理局基金项目(04DSZP30)
