摘要
采用无菌溶液培养试验，研究了水稻对３种氨基酸（甘氨酸、谷氨酸和精氨酸）吸收的动力学参数，以及无机氮（ＮＨ＋４和ＮＯ－３）对氨基酸吸收动力学参数的影响。结果表明，水稻对３种氨基酸的吸收都符合米氏方程。其吸收具有主动吸收的特征。水稻对３种氨基酸吸收动力学参数因氨基酸的种类而异。Ｋｍ值为精氨酸（Ａｒｇ）＜谷氨酸（Ｇｌｙ）＜甘氨酸（Ｇｌｕ），而３种氨基酸的Ｉｍ值相似。３种氨基酸约在０１ｍＭ浓度时吸收基本达到最大。从试验结果可以看出，由于Ａｒｇ具有最小的Ｋｍ值，故其与水稻根系的亲和力最强。添加ＮＨ＋４后，水稻对氨基酸的吸收仍符合米氏方程，ＮＨ＋４对３种氨基酸的Ｉｍ值无显著影响，但改变了氨基酸吸收的Ｋｍ值，Ａｒｇ和Ｇｌｙ的Ｋｍ值增加，而Ｇｌｕ降低。添加ＮＯ－３后，水稻吸收氨基酸符合米氏方程。ＮＯ－３对精氨酸的吸收动力学参数没有实质性的影响，对其它两种氨基酸的Ｉｍ值也几乎没有影响，但对Ｋｍ值有较大的影响。对Ｇｌｕ的Ｋｍ值有显著的降低。水稻不同种氨基酸吸收的差异。
A sterilized water culture was conducted to study the kinetics of Glu, Gly and Arg absorption by rice seedlings The results showed that the absorption of the tested three amino acids fitted for the Michlaes equation No significant difference in Im was found among the three aminao acids The Km was followed in this order: Arg<Gly<Glu The addition of NH4+ did not affect Im, but increased the Km of Arg and Gly and decreased that of Glu The addition of NO3- did not affect the Im and the absorption of Arg, but decreased the Km of Glu The difference in kinetic paramters of the three amino acids might be concerned with their electric characteristics
出处
《浙江农业学报》
CSCD
1997年第2期62-65,共4页
Acta Agriculturae Zhejiangensis
基金
浙江省自然科学基金
关键词
水稻
秧苗
氨基酸
吸收
rice seedling
amino acids
absorption
Michlaes equation
