摘要
目的评价神经根修复时间对脊髓前角运动神经元存活的影响。方法采用大鼠颈_(5-7)神经根性撕脱伤和椎孔外切断实验动物模型,根据动物神经根损伤类型和损伤后修复时间分为A、B、C、D四组,每组16只。A、C组分别将撕脱与切断的神经根即刻回植入脊髓和于椎孔外直接吻合,B、D组将撕脱和椎孔外切断的神经根旷置3周后再重复上述手术。分别于神经根撕脱和椎孔外切断术后3周、3个月、6个月取材。应用组织病理活检、免疫组化技术及神经示踪技术,对神经中枢及吻合口下段神经干检查,观察脊髓病理改变和轴突再生情况,进行A、B组和C、D组间对比。结果臂丛神经根性损伤即刻修复组脊髓前角运动神经元和尼氏体数目、形态、减少程度及周围再生神经纤维数目、发育程度均优于损伤后3周修复组。结论神经根早期修复对脊髓前角运动神经元有明确的保护作用,对外周神经再生有促进作用。
Objective To measure the effect on rat＇s spinal neuron flow according nerve roots repair time. Methods We adopted the experimental rats on the root avulsion and extravertebral foramen nerve root divison of C5-7. We divided them into four groups, in each which there were 16 rats, according the type of nerves root injury and repair time. Group A, C： the avulsed roots were reimplanted into the spinal cord and the transected roots were sutured to the proximal stump immediately. Group B,D： the avulsed roots and the transected roots were reimplanted into the spinal cord or were sutured to the proximal stump in delayed 3 weeks each with 16 rats. At the different time point（3 weeks, 3 months, 6 months）, through pathological examination and immunohistological techniques and nerve tracing techniques, we examined the spinal cord and distal nerve trunk in order to observe the pathologic changes and axonal regeneration. Results Group A, C were much better than group B,D in the number, the conformation and the degree of abatement of spinal motoneurons and nissl body. It is the same on the number and the development level of regenerating nerve fiber. Conclusion It had the advantage of neuronal protection and nerve regeneration that reparing the injured nerve roots early after nerve roots injury.
出处
《中华显微外科杂志》
CSCD
北大核心
2007年第4期277-280,I0010,共5页
Chinese Journal of Microsurgery
基金
吉林省科技厅资助项目(200505232)
