摘要
目的:探讨慢性肾功能衰竭(chronic renal failure,CRF)对大鼠阴茎海绵体缝隙连接蛋白43表达的影响。方法:应用SD雄性大鼠36只,同期行5/6肾脏切除术,建立CRF动物模型。随机分为假手术组(NCRF组,n=6)、CRF模型组(CRF组,n=30),分别于12周注射阿朴吗啡(APO)80μg/(kg.d)后观察大鼠阴茎勃起情况,应用Western blot方法检测大鼠阴茎海绵体缝隙连接蛋白43(connexin 43,CX43)表达。结果:NCRF组死亡1例,CRF模型组死亡5例。CRF组阴茎勃起率为28%(7/25),NCRF组阴茎勃起率100%(5/5),两组比较差异有统计学意义(P<0.05)。CRF组阴茎勃起次数为(1.0±0.0)次,NCRF组阴茎勃起次数(2.2±0.8)次,两组比较差异有统计学意义(P<0.05)。CRF组大鼠阴茎海绵体组织CX43(0.18±0.08)低于NCRF组(0.53±0.27),两组比较差异有统计学意义(P<0.01)。结论:CRF明显降低大鼠阴茎勃起功能。CRF降低大鼠阴茎勃起功能与大鼠阴茎海绵体CX43表达减少密切相关。
Objective： To investigate the effect of chronic renal failure （CRF） on connexin 43 expression in the penis of rats. Methods： CRF models were established by a resection of 5/6 kidney from male sprague-dawley （SD） rats in one stage. Rats of the control group （ NCRF group, n = 6） and the experimental group （ CRF group, n = 30） were subjected to a low dose （80 μg/kg·d） of apemorptfine （APO） at the 12th week after resection. The correspondent penile erection was determined. Expression of eonnexln 43（ex43） in the rat corpus cavemesum was also determined by the Western blot method. Results： One rat died in the NCRF group and five in the CRF group. The penile erection ratio of the CRF group was 7/25 and of the NCRF group was 5/5, which presented a significant difference（ P 〈 0.05）. The average penile erection frequency of the CRF group was（1.0±0.0）and of the NCRF group was（2.2 ± 0.8）, which was also significantly different （ P 〈 0.05）. Expression of ex43 in the rat corpus eavemesum of the CRF group was lower than that of the NCRF group [（0.21 ±0.07 ） vs（0.40±0.08）] （P 〈0.01）. Conclusion： CRF significantly reduces the erectile function of the rots, CRF-related ED bears a correlation with the decrease in expression of ex43 in the rat corpus eavemesum.
出处
《山东大学学报（医学版）》
CAS
北大核心
2007年第8期778-780,共3页
Journal of Shandong University:Health Sciences
基金
山东省卫生科技发展计划资助课题(2003-40)
