摘要
目的观察大鼠神经干细胞(NSC)植入帕金森病(PD)模型大鼠的脑纹状体后的存活、分化及功能状态。方法体外分离培养NSC,单克隆培养,免疫细胞化学检测多向分化潜能和特异性标记nestin。建立PD大鼠模型,纹状体内植入DAPI标记的NSC,检测6-羟基多巴胺(6-HHDA)诱发的旋转行为,荧光检测标记细胞的分布情况,免疫细胞化学和高效液相色谱检测细胞分化和神经递质含量。结果培养的NSC表达nestin,具有自我更新和多向分化能力;NSC植入PD模型大鼠纹状体后大鼠诱导旋转行为显著改善(P<0·05);NSC在脑内迁移,并在纹状体内形成少量酪胺酸羟化酶(TH)阳性细胞、纤维;植入后纹状体内多巴胺(DA)及其代谢产物含量上升,2个月时分别增加3·6倍和2·8倍,4个月时增加3·4倍和2·4倍(P<0·01)。结论神经干细胞在体外大量扩增后植入PD脑内能长期存活并分化、分泌神经递质,从而部分改善PD症状。
Objective To investigate the survival, striatum of Parkinson disease rat model. Methods differentiation and action of neural stem cell transplanted into Neural stem cells were isolated and cultured. The character of self-renewing was confirmed by technique of single cell clone culture. The ability of multi-directional differentiation and specific antigen-nestin was showed by immunocytochemistry（ICC）. PD rat models were established, and the DAPI-labelled neural stem cells were transplanted into striatum. The 6-HHDA induced rotary behavior was measured, distribution and migration of the cell were checked by fluoresce activating. ICC and HPLC are used to see its differentiation and action of secrete neurotransmitter dopamine （DA） and it＇s metabolites. Results The cultured stem cell express nestin and have the ability of self-renewing and multi-directional differentiation. 6-HHDA induced rotation decreased after transplantation of NSC into the PD rat striatum（P 〈 0. 05 ） o NSC migrate widely in the recipient and form small member of TH cell, fibre in striatum. Content of DA and its metabolite （dopac） of rat＇ s striatum after transplanting increased. Conclusion Transplanted neural stem cell can survived in brain of PD rat and differentiate to cells suit to microcircumstances by excreting neurotransmitter and improve the symptom of PD.
出处
《基础医学与临床》
CSCD
北大核心
2007年第8期841-845,共5页
Basic and Clinical Medicine
基金
国家重点基础研究发展规划项目(grants from National Key Project for Basic Research of China)(001CB509906)
国家高技术研究发展项目(State High Technology R &D Project of China)(2002AA205051
2001AA216151)
关键词
神经干细胞
细胞分化
帕金森病
neural stem cell, cell differentiation, Parkinson＇s disease
