摘要
以30%和95%的穿心莲内酯为实验原料,采用超临界CO2萃取结晶法考察了不同压力下穿心莲内酯在结晶板上的分布规律,同时也考察了晶体的晶型和红外光谱的变化规律。研究证实:高效液相色谱分析,不同压力下穿心莲内酯在结晶板上都按纯度梯度结晶分布;X射线衍射分析,压力越高,晶体越趋于向比较单一的晶面上择优生长;红外光谱分析,压力的变化,并没有引起穿心莲内酯化学结构的改变。
With the new technique of supercritical carbon dioxide extraction and crystallization, the experimental materials of 30% and 95% andrographolide were used to investigate the effect of different pressure on crystal distribution on crystallization board, what＇s more, the effect of X-ray diffraction and infrared ray spectrum on crystal was tested. The results showed that andrographolide was crystallized on crystallization board in purity gradient with high performance liquid chromatography; the high- er the pressure, the higher the crystallization ratio. In addition, the crystal was inclining to directional growth; the chemical structure of andrographolide was not changed with the variety of pressure with infrared ray spectrum analysis.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2007年第8期1657-1660,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(29976008)
教育部科技创新工程重大项目培育基金项目(704027)资助
关键词
超临界CO2
萃取结晶
穿心莲内酯
高效液相色谱
X射线衍射
红外光谱
Supercritical CO2
Extraction and crystallization
Andrographolide
High performance liquid chromatography
X-ray diffraction
Infrared ray spectrum
