摘要
激发波长和发射波长是分子荧光光谱测量的重要参数,它的选择影响分子荧光光谱的测量以及其后续研究的精确度。由于如HITACHI F-4500荧光分光光度计等一些光谱仪器的测量精度达不到一些光谱分析技术的要求以及测量数据的有限,在实际工作中,利用已测量的光谱数据,通过插值生成所需要的光谱数据,就成为一种有效的解决方法。因此,文章使用指示克立格法对一定体积分数的乙醇溶液的最佳激发波长和最佳发射波长的确定进行了研究。基于指示克立格法求取的最佳激发波长和最佳发射波长分别是226.9和337.1 nm,经验证其值是准确的。此外,文章采用相对标准偏差对插值结果进行了评价。结果表明在分子荧光光谱测量中基于指示克立格法确定最佳激发波长和最佳发射波长是可行的,这为最佳激发波长和最佳发射波长的确定提供了一种新的方法。
Excitation wavelength and emission wavelength are vital parameters of molecular fluorescence spectral measurement, whose choice influences the precision of molecular fluorescence spectral measurement and its subsequent study. Because measure- ment precision of some spectral instrument such as fluorescence spectrometer HITACHI F-4500 can not reach the requirement of spectral analytical technique, and the measurement data is finite, in practical work, the required data are obtained from measurement data using interpolation, which becomes an effective solving method. Therefore, the present paper studies the confirmation of certain volume percentage ethanol solution＇s optimal excitation wavelength and optimal emission wavelength using indicator Kriging method： the optimal excitation wavelength and optimal emission wavelength based on indicator Kriging method are 226.9 and 337. 1 nm respectively, with verification. Furthermore, the interpolation result is valuated using relative standard deviation. These results indicate that it is feasible to confirm the optimal excitation wavelength and optimal emission wavelength based on indicator Kriging method in the molecular fluorescence spectral measurement, which supplies a novel method for the confirmation of optimal excitation wavelength and optimal emission wavelength.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2007年第8期1476-1479,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(60378018
60578026)
医学光电科学与技术教育部重点实验室(JYG0502)资助
关键词
最佳激发波长
最佳发射波长
指示克立格法
相对标准偏差
Optimal excitation wavelength
Optimal emission wavelength
Indicator Kriging method
Relative standard deviation
