摘要
目的:以改性聚乳酸为细胞外基质网架,探索利用其构建复层组织工程皮肤的可行性。方法:取出生24h以内的Wistar大鼠背部皮肤,以Dispase-trypsin双酶消化分离培养表皮角质形成细胞;以Ⅰ型胶原酶消化分离培养真皮成纤维细胞。采用盐析法制备机械性能得到部分改进的聚乳酸多孔泡沫支架,向支架接种真皮成纤维细胞(密度为3.0×105cell·mL-1)和表皮角质形成细胞(密度为5.0×105cell·mL-1),体外培养1周,构建复层组织工程皮肤,并取培养的皮肤进行切片检测。结果:复层组织工程皮肤在结构上与正常皮肤相似,具有真皮、表皮双层结构。改性聚乳酸网架上有双层细胞生长,生长的细胞与网架接触,并且在其表面形成较为明显而连续的细胞层。结论:双醛淀粉作为良好的增柔剂在改善聚乳酸网架机械性能的同时,也使其具有良好的细胞相容性,不影响细胞的生长增殖和代谢,可以进一步用作组织工程皮肤的支架材料。
Objective To explore the possibility of reconstruction of tissue engineering artificial skin with modified poly lactic acid （PLA） . Methods Fibroblasts （FB） and keratinocytes （KC） from new born rats isolated by treatment of Dispase-trypsin and coUaganase were cultivated. Tissue engineering artificial skin was reconstructed by planting subcultured FB （3.0×10^5cell·mL^-1 ） and KC （5.0×10^5cell·mL^-1 ） separately onto the modified PLA, which was made by special ways and it＇s mechanism function was improved, and cultivated for one week. Results The artificial skin was composed of epidermis and dermis and similar with natural skin. The skin consisted of FB and KC was at various proliferation and differentiation stages. FB and KC increased on the surface of PLA. Conclusion Dialdehyde starch （DAS） not only improves the mechanism function of PLA, but also has good effect on cells. And it doesn＇ t affect the growth and metabolism of cells. So it is feasible as scaffold of tissue engineering artificial skin.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2007年第4期719-722,F0003,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅重点项目资助课题(20050401-2)
