摘要
为探讨即早反应基因（ｉｍｍｅｄｉａｔｅｅａｒｌｙｇｅｎｅｓ，ＩＥＧｓ）在神经损伤过程中的变化规律，用原位分子杂交和ＰＡＰ免疫组织化学方法观察了大鼠脊髓内注射红藻氨酸（ｋａｉｎｉｃａｃｉｄ，ＫＡ）后１ｈ至１４ｄ的不同时间点，Ｌ１～３脊髓腹、背角神经元中ｃ－ｆｏｓｍＲＮＡ和Ｆｏｓ免疫阳性信号的变化。结果表明，ＫＡ致脊髓损伤后２～８ｈ脊髓腹角运动神经元中ｃ－ｆｏｓｍＲＮＡ的表达和Ｆｏｓ免疫组织化学阳性反应明显增强，１２ｈ恢复至正常水平，伤后３ｄ又明显增强；而脊髓背角神经元内仅在伤后２～６ｈ明显增加。提示ＫＡ对脊髓神经元的选择性毒性作用；运动神经元ｃ－ｆｏｓ表达的再次增强与ＫＡ所致神经元的不可逆变性有关，亦可看作是神经元损伤后死亡的先兆之一。
For studing the relationship between the immediate early genes( IEGs) and nerve injury, the expressions of c fos mRNA and Fos in rat spinal cord neurons was examined by in situ hybridization using photobiotin avidin gold silver stained c fos cDNA probe and PAP immunohistochemical method during 1h 14d after intraspinal cord injection of kainic acid (KA). Image analysis showed that the optical densities of these hybridization signals with c fos cDNA probe and Fos IR signals increased significantly in L 1～3 ventral motoneurons of KA treatrment rat, starting from 2 hour after KA injection, reaching to a maximum in 4 and 6 hours, and retured to control level 12 hours post injury. These were followed by a second inerease by 3rd days in ventral motoneurous. However, a dramatic inerease of expresions of c fos mRNA and Fos in dorsal horn neurons were observed during 2 to 6 hours after KA injection .The results indicated that KA is a selective neurotoxin for spinal cord neurons. For motoneurons, second increase of c fos expression was related with neurodegeneration by KA treatment,and was thought to be an early harbinger of neuronal death.
出处
《解剖学报》
CAS
CSCD
北大核心
1997年第2期151-156,共6页
Acta Anatomica Sinica
关键词
脊髓损伤
红藻氨酸
C-FOS
MRNA
FOS
原位杂交
Kainic acid
Neurontoxin
Spinal cord
c fos mRNA
Fos
In situ hybridization
Immunohistochemistry
