摘要
应用荧光原位杂交技术（FISH）和免疫组化法观察50例肺癌中Her－2／neu基因扩增及其蛋白表达状况、组织学分级和淋巴结转移的关系。结果有Her－2／neu基因扩增者共2例（4％）。有Her－2／neu基因蛋白表达共12例（24％），其中鳞癌1例，肺泡细胞癌4例，腺癌6例，神经内分泌癌1例。肺癌组织学分级Ⅰ级与Ⅱ、Ⅲ级间该基因蛋白表达均有明显差异（P＜0．05），有肺门或支气管淋巴结转移与无淋巴结转移蛋白表达亦有明显差异（P＜0．05）。研究结果表明Her－2／neu基因编码的P185KD蛋白在肺癌分化及转移过程中起重要作用。
In the present study,we examined Her-2/neu protooncogene amplification and expression in 30 cases of primary lung cancer using fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC), and the relationship between amplification and expression of Her-2/neu and the histological grading and hilar peribronchial lymph node metastasis were evaluated. Results showed that the Her-2/neu gene amplification was found in 2 of 50 cases. Her-2/neu gene expression was identified in 12/50 (squamous cell carcinoma 1,bronchioalveolar carcinoma 4,adenocarcinoma 6 .neuroendocrine cancer 1 Marked differences were noted between histological grades Ⅱand Ⅲ(P＜0. 05),and between the cases with lymph node metastasis and without metastasis (P＜0. 05 ),as well. Our results indicate that Her2/neu gene and the encoded protein play an important role in the mechanism of differentiation and metastasis of human lung cancer.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
1997年第6期452-455,共4页
Chinese Journal of Clinical Oncology
