摘要
基于KIO4在H2SO4介质和沸水浴中可氧化偶氮氯膦Ⅲ褪色，而蛋白质能灵敏地阻抑该氧化褪色反应，建立了测定痕量蛋白质的新方法。蛋白质测定范围为0-6μg/mL，方法的检出限为2.9×10^-10g/L，回收率为96%-109%。测定了动力学参数，并对反应机理做了研究。该方法可用于实际样品的测定。
A new method for the detennination of trace bovine serum albumin （BSA） was proposed. It was based on the inhibitory effect of protein on discoloring reaction between KIO4 and chlomphosphonazo Ⅲ in dilute stulfuric acid solution at 100℃. The calibration graph is rectilinear from 0 to 6 μg/mL for BSA and the detection limit is 2.9 × 10^-10 g/L. Kinetic parameters and reaction mechanism have been studied. This method is simple and sensitive. It has been applied satisfactorily to the detennination of BSA in milk samples.
出处
《分析试验室》
CAS
CSCD
北大核心
2007年第8期37-40,共4页
Chinese Journal of Analysis Laboratory
基金
广西教育厅科研(200508135)项目资助
