摘要
本研究探讨急性白血病和正常骨髓基质细胞中连接蛋白43(connexin 43, Cx43)的表达及细胞间隙连接通讯(gap junction intercellular communication, GJIC)功能的变化及二者细胞通讯功能之间的差异。体外培养正常及急性白血病骨髓基质细胞,采用细胞免疫组织化学方法及计算机灰度检测观察二者之间Cx43表达的变化,采用细胞划痕染料传输技术比较两者之间GJIC功能的差异。结果显示,体外培养的急性白血病骨髓基质细胞Cx43的表达较正常骨髓基质细胞Cx43的表达明显减低,GJIC功能减弱。结论:急性白血病骨髓基质细胞间通讯功能较正常骨髓基质细胞明显减低。
This study was purposed to investigate the connexin 43 （ Cx43 ） expression level in acute leukemia bone marrow stromal cells （ABMSCs） and normal bone marrow stromal cells （NBMSCs）, and to explore the difference in communicating functions between these cells. The Cx43 expression levels of ABMSCs and NBMSCs were detected by using immunohistochemistry and computer gray scale assay, and the difference of gap junction intercellular communication（GJIC） was examined through dry transfer technique. The results showed that expression level of Cx43 in ABMSCs was lower than that in NBMSCs and its function of GJIC in ABMSCs was also weaker than that in NBMSCs. It is concluded that cell-cell communication function is lowered in ABMSCs.
出处
《中国实验血液学杂志》
CAS
CSCD
2007年第4期679-682,共4页
Journal of Experimental Hematology
基金
国家自然科学基金项目资助项目
编号30500220
