摘要
应用非同位素PCR－SSCP方法分析30例食管瘤患者癌组织及相应癌旁组织p53基因第5～9外显子，结果发现8例癌组织存在p53基因突变；其中的4例为p53基因杂合性丢失（LOH）。部分癌旁组织亦检测到p53基因突变。实验结果表明，p53基因突变可能是食管癌癌变的早期遗传学损害，对食管癌的发生起重要作用。
30 cases of human squamous ccll carcinoma were examined by non -isotope PCR-SSCP for the presence of p53 mutations in exons 5, 6, 7, 8 and 9 in tumorous tissues and nontumorous tissues rcspcctlvely. Mutations werc detected at exons 5~8 of the p53 gene in 8 tumorous tissues as wcll as in some nontumorous tissues. Of all the eight cases that har boured p53 genc mutations, four wcre ohscrved to retaln LOH on the p53 locus. These results suggested that the mutation of the p53 gene could be an early genetic aberration in the car clnogenesis of squamous cell carc1nornas of the esophagus, and might play an important role in the occurrence of the tumor.
出处
《汕头大学医学院学报》
1997年第1期1-4,共4页
Journal of Shantou University Medical College
关键词
P53基因
基因突变
食管肿瘤
esophageal squamous cell carcinoma p53 gene mutation
