摘要
[目的]应用黄芪注射液治疗支气管哮喘模型大鼠,对黄芪注射液治疗哮喘的机理进行初步的研究。[方法]建立支气管哮喘大鼠模型,应用流式细胞计数仪和酶联免疫吸附试验检测黄芪注射液对支气管哮喘模型大鼠的免疫调节作用,并与模型组和正常对照组作比较。[结果]黄芪注射液治疗组大鼠CD4+T细胞百分率及CD4+/CD8+比值均显著高于正常对照组和模型组(P﹤0.01,P﹤0.05),治疗组CD8+T细胞百分率显著低于模型组(P﹤0.05);模型组大鼠脾脏淋巴细胞体外培养分泌IL-2、IL-4水平显著高于对照组和治疗组(P﹤0.01)。[结论]黄芪注射液可以有效改善支气管哮喘模型大鼠的临床症状、恢复大鼠机体的免疫平衡调节作用,为黄芪注射液的进一步临床应用提供了实验依据。
[Objective] To study the effects of Huangqi injection on Bronchial Asthma Model rat. [Methods] Bronchial Asthma Model was set up, and flow cytometry and ELISA were employed to detect the changes of inanune functional parameters to compare with the results of other two groups. [Results] The proportion of CD4^＋ T cell and the ratio of CD4^＋/CD8^＋ were obviously higher in treatment group than control group and model group （P 〈 0.01, P 〈 0.05 ）. The proportion of CD8^＋ T cell was significandy lower in treatment group than model group （P 〈 0.05）. The levels of IL-2, IL-4 were obviously higher in model group than control group and treatment group （P 〈 0.01）. [Conclusion] Huangqi injection can improve the clinical symptom, help to remain immunologic balance of Bronchial Asthma Model rat, which supplies experimental evidence for further clinical application of Huangqi injection.
出处
《现代预防医学》
CAS
北大核心
2007年第15期2845-2846,共2页
Modern Preventive Medicine
