摘要
目的:研究原儿茶醛缩氨基硫脲铜(II)配合物(PTC)对高脂血症模型大鼠血脂、主动脉组织形态学及肝组织抗氧化活性的影响.方法:60只SD大鼠随机分为6组(每组10只),即正常对照组、高脂血症模型组、药物对照组(即费非诺贝特组,0.04g/kg),PTC小剂量组(0.005g/kg),PTC中剂量组(0.05g/kg),PTC大剂量组(0.25g/kg).在以高脂饲料喂养5wk开始,正常对照组和高脂血症模型组给以5g/L羧甲基纤维素钠水溶液10mL/kg体质量灌胃.其他实验组分别给以5g/L羧甲基纤维素钠水溶液配成药物混悬液灌胃,每日一次.实验到10wk后全自动生化分析仪测定各组动物血脂,HE染色做病理切片观察大鼠主动脉形态学变化,同时测定肝组织超氧化物歧化酶(SOD),过氧化氢酶(CAT)活性及抑制羟自由基(.OH)的能力.结果:原儿茶醛缩氨基硫脲铜(II)配合物能使模型大鼠肝组织清除羟自由基(.OH)的能力提高(P<0.01),SOD的活性增强(P<0.01或0.05);各剂量组均有降低血清胆固醇和提高血清高密度脂蛋白(HDL-C)含量的趋势,但无统计学意义.结论:原儿茶醛缩氨基硫脲铜(II)配合物在实验动物体内的确有清除超氧阴离子自由基(O2.-)和羟自由基(.OH)的双功能作用.
AIM： To investigate the effects of protocatechualde- hyde- thiosemicarbazone-copper（II） complex （PTC） on the serum lipids, the aortic tectology and the activity of antioxidation in liver tissues of the hyperlipemia model rats. METHODS： Sixty healthy SD rats were randomly divided into 6 groups（ 10 rats per group）, including control group, hyperlipidemia model group, Fenofibrate drug group（0.04 g/kg） and 3 PTC groups, namely small dosage of PTC （0. 005 g/kg） group, moderate dosage of PTC （0.05 g/kg）group, large dosage of PTC （0.25 g/kg） group. Control group was fed by ordinary forage;the other groups were fed by high-lipid forage. From the 5th week, besides the above feeding, control group and model group were administrated intragastrically 5 g/L Carboxy Methyl Cellulose （CMC）; Fenofibrate drug group and 3 PTC groups were respectively administrated intragastrically suspension with Fenofibrate and CMC, and suspension with PTC and CMC. After 10-week feeding, the levels of serum lipids were determined; aortic histomorphological changes were observed by the pathological section with the HE staining; the activities of superoxide dismutase（SOD） and catalase （ CAT）, and the restraining ability to hydroxyl free radical（· OH） in the liver tissues were measured. RESULTS： PTC could restrain OH （P 〈0.01 ） and enhance the activities of SOD （P 〈 0.01 or P 〈 0.05 ）. Every PTC group had tendencies to decrease cholesterol and to increase high-density lipoprotein cholesterol, but there had no significance in statistics（P 〉0.05）. CONCLUSION： PTC can certainly eliminate the superoxide free radical （ O2^· - ） and · OH in experimental animals in vivo.
出处
《第四军医大学学报》
北大核心
2007年第14期1253-1256,共4页
Journal of the Fourth Military Medical University
基金
陕西省卫生厅科研基金项目(04D15)
关键词
原儿茶醛缩氨基硫脲铜(Ⅱ)配合物
自由基清除剂
超氧化物类
羟自由基
protocatechualdehyde-thiosemicarbazone copper （ Ⅱ ） complex （ PTC ）
free radical scavengers
superoxides
hydroxyl radical
