摘要
对恒河猴进行二次接种H5N1亚型禽流感病毒试验,并对二次接毒的结果进行观察,评估初次接毒对恒河猴二次接毒效果的影响。首次接种试验中,3、4、5号恒河猴用环甲膜穿刺注射方法接种含有H5N1亚型禽流感病毒的尿囊液,6号猴接种不含病毒的尿囊液。90d后,再次用环甲膜穿刺注射方法二次接种,4、5、6号猴接种7mlTCID50浓度为104.875病毒的尿囊液,3号猴接种7ml不含病毒的尿囊液。进行抗体等检测,并分别于72h无痛处死3、4、6号猴,第7d无痛处死5号猴,进行肺的病毒检测及病理观察。结果显示,3、4、5号猴至试验结束时体内依然有较高的抗H5N1亚型禽流感病毒抗体水平,6号猴没有抗体;通过RT-PCR以及免疫组化染色进行病毒检测,均只在6号猴肺部检出病毒,且6号猴肺部病理损伤最为严重。由此可以得出初步结论:在初次感染H5N1亚型禽流感病毒后90d,临床症状已基本恢复正常的恒河猴体内仍然有较高水平的抗体,此时,恒河猴抵抗H5N1亚型禽流感病毒二次感染的能力显著提高。
Rhesus monkeys with high specific H5N1 antibody were inoculated the second time with H5N1 virus, the result of the second time H5N1 inoculation and the effect of first time H5N1 inoculation on second inoculation was evaluated. Monkeys of NO. 3, NO. 4, NO. 5 were inoculated with H5N1 allantoic fluid and NO. 6 with noninfectious allantoic fluid by intratracheal thyrocricoid puncture. Three months later, NO. 4, NO. 5, NO. 6 monkeys were infected with 7ml TCID50 10^4.875 H5N1 allantoic fluid and NO. 3 monkey with 7ml noninfectious allantoic fluid at the same time by the same method. Clinical symptoms were recorded and antibody response was detected by ELISA. NO. 3, NO. 4, NO. 6 monkeys were killed after 72h post infection and NO. 5 monkey was killed after 7 days post infection. Pathologic changes of the infected monkeys＇ lung were examined by HE staining,immunohistochemistry and the virus in lung was detected by RT-PCR. Results showed that NO. 3, NO. 4, NO. 5 monkeys still retained high level of specific antibody, H5N1 virus only could be detected in NO. 6 monkey＇s lung by immunohistochemistry and RT-PCR ,and the lung of NO. 6 monkey injured worst . It can be concluded that Rhesus monkeys inoculated with H5N1 avain influenza A virus at the first time could retain a high level of specific antibody in 90 days and the clinical symptom had almost recovered, the ability of Rhesus monkeys to resist second infection of H5N1 virus was enhanced no.tably at that moment .
出处
《病毒学报》
CAS
CSCD
北大核心
2007年第4期282-286,共5页
Chinese Journal of Virology
基金
广东省农业攻关项目2004B20201028
关键词
禽流感病毒
恒河猴
接种
avain influenza A（H5N1） virus
Rhesus monkey
inoculation
