摘要
目的：研究α干扰素（ＩＦＮ－α）对白细胞介素２（ＩＬ－２）激活的淋巴细胞中穿孔素和颗粒酶表达的影响。方法：４小时标准５１Ｃｒ释放实验检测ＮＫ和ＬＡＫ活性，ＡＢＣ酶标法检测颗粒酶Ｂ的表达，溶血法测定穿孔素活性，ＢＬＴ法测定颗粒酶Ａ的表达。结果：ＩＦＮ－α能增强ＩＬ－２诱导的外周血淋巴细胞中ＮＫ和ＬＡＫ细胞活性。ＩＬ－２和ＩＦＮ－α分别作用于淋巴细胞１天即可增加穿孔素活性，二者联合可使活性进一步增强（Ｐ＜０．０５），于培养第３天，ＩＬ－２和联合组的穿孔素仍有高表达，但ＩＦＮ－α组则下降至对照组水平。ＩＦＮ－α和ＩＬ－２单独或联合对淋巴细胞颗粒酶Ａ和Ｂ的表达无影响。结论：ＩＦＮ－α增加ＩＬ－２激活淋巴细胞的细胞毒作用可能与其增加淋巴细胞穿孔素表达相关。
Objective:To study the effect of IFN α on the expressions of perforin and granzymes in IL 2 activated lymphocytes.Methods:NK and LAK activities were assayed by 4 hour standard 51 Cr release test,the activity of perforin was detected by hemolysis method,expression of granzyme B was measured by ABC immunohistological method,expression of granzyme A was measured by BLT method.Results:IFN α significantly augmented the activities of NK and LAK in IL 2 activated peripheral blood lymphocytes(PBL) after 1 day culture.Perforin activity in lymphocytes was increased after 1 day exposure to IL 2 or IFN α,and was enhanced when exposed to the combination of IL 2 and IFN α.After 3 day culture,the perforin activity remained high in lymphocytes activated by IL 2 alone or in combination with IFN α,while declined to control level in IFN α exposed group.IL 2 and IFN α alone or in combination had no effect on expression of granzyme A and B.Conclusion:IFN α enhances the cytotoxicity of lymphocytes activated by IL 2.The mechanism might be that IFN α upregulates the perforin expression.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1997年第6期295-298,共4页
Chinese Journal of Hematology
