摘要
利用化学致突变物甲基丙烯酸环氧丙酯体外诱导人胚肺成纤维细胞，使之发生转化，分离转化细胞克隆。经ＰＣＲ特异性扩增ｐ５３基因外显子第５和第８，银染单链构象多态性（ＳＳＣＰ）分析，结果表明转化细胞中ｐ５３基因外显子第８发生突变，揭示出ｐ５３基因在化学致突变物诱导细胞转化中起着重要作用。
The human embryonic lung fibroblasts transformation was induced by chemical mutant Glycidyl Methacrylate (GMA) in vitro. Transformed clonies were isolated and then exon 5 and exon 8 of p53 gene were specifically amplified by polymerase chain reaction. Amplified fragments were detected by using silver stained single strand conformation polymorphism analysis. The results showed that exon 8 altered in the transformed cells, indicating that p53 gene played an important role in the human embryonic lung fibroblasts transformation induced by GMA .
出处
《卫生研究》
CAS
CSCD
北大核心
1997年第3期159-161,共3页
Journal of Hygiene Research
基金
国家自然科学基金
卫生部基金
关键词
化学致突变物
细胞转化
P53基因
PCR
chemical mutant
cell transformation
p53 gene
silver stained
Polymerase Chain Reaction Single Strand Conformation Polymorphism analysis.
