摘要
选用２月龄Ｗｉｓｔａｒ大鼠，在距梨状肌下孔０．５ｃｍ处切断右侧坐骨神经，近侧断端套入硅胶管，然后分别注入２０μｌＳｃｈｗａｎｎ细胞县液、层粘蛋白２０μｇ／ｍｌ和生理盐水。４周后观察到Ｌ４（Ｌ５）节神经元存活率分别是９０％、８０％和７２％（８８％、７３％和５３％）。
After sciatic nerve resetion, we put the normal saline (NS), laminin (LN) and the cultured Schwann cells(SCs) respectively into the silicon chamber, which was sutured to the proximal end of sciatic nerve. At 4 weeks, the survival rates of the neurons in L4(L5) dorsal root ganglion (DRG) were 72%, 80% and 90% (53%, 73% and 88%) respectively. The acid phosphatese (ACP) activity increased while the cholinesterase(ChE) activity decreased significantly in the NS group. LN and SC proved to decrease ACP activity and increased ChE activity. These results showed that LN and SCs had protective effect on the sensory neurons in vivo, suggesting that LN and SCs are conductive to the regeneration of peripheral nerves and the recovery of their function.
出处
《解剖学杂志》
CAS
CSCD
北大核心
1997年第2期136-140,共5页
Chinese Journal of Anatomy
基金
国家自然科学基金资助No.39370246
