摘要
应用免疫组织化学ABC法结合显微图像定量分析,研究了神经激肽A免疫反应在哮喘豚鼠延髓中的分布及其变化.结果表明:神经激肽A免疫反应细胞纤维或终末分布于豚鼠的延髓的弧束核区.腹外侧面浅表区,三叉神经脊束核区及网状结构等区域.此外,哮喘豚鼠延髓孤束核及腹外侧部浅表区内神经激肽A阳性免疫反应物的平均密度分别为49.2%和41.6%;它们的平均灰度值分别为129.6和110.5,与对照组(平均密度31.7%和28.8%;平均灰度值:183.7和165.2)比较均有显著性差异(P<O.01).结果提示:延髓内神经激肽A可能参与哮喘发作的神经病理生理机制,是其发作的重要因素之一.
Alteration of neurokinin A-like immunoreactivity (NKA-LI) in the medulla of experimental asthmatic guinea pigs was studied by using the immunocytochemical ABC( avidin biotin-peroxi-dase complex) method combined with image quantitative analysis. The average density of NKA-LI neurons and nerve fibers of the nucleus of the solitary tract (SOL) region and the ventrolateral superficial area of the medulla (VMS) of asthmatic guinea pig was 49. 2% and 41. 6% respectively, while the average grey level of above regions was 129. 6 and 110. 5 respectively, which showed that the content of NKA in the experiment animal significantly increased (P<0. 01) as compared with that of the control group (average density: 31. 7% and 28. 8%, average grey level: 183. 7 and 165. 2 in SOL and VMS). The results suggest that NKA in the central nervous system is related to the respiratory modulation and plays an important role in the pathogenesis of asthma.Our results may provide a theoretical basis for the treatment of asthma with NKA antagonist or inhibitor of its receptors.
出处
《解剖学杂志》
CAS
CSCD
北大核心
1997年第2期128-132,共5页
Chinese Journal of Anatomy
基金
国家自然科学基金资助项目.批准号 39270338
关键词
神经激肽A
延髓
哮喘
neurokinin A
nucleus of solitary tract
medulla oblongata
asthma
guinea pig
