摘要
目的探讨原发性肝癌患者Th1/Th2免疫反应状态。方法用ELISA和RT-PCR方法检测24例原发性肝癌患者、24例健康人群外周血中Th1/Th2相关细胞因子浓度及mRNA表达。结果原发性肝癌患者外周血Th1相关细胞因子IL-2、IFN-γ的浓度与mRNA表达均明显低于健康人群(P<0.05),而Th2相关细胞因子IL-4、IL-10的浓度与mRNA表达均明显高于健康人群(P<0.05)。结论原发性肝癌患者Th1/Th2细胞亚群存在着Th1向Th2的漂移,并可通过这一机制逃避机体免疫监视。
Objective To investigate the immunological reaction state of Th1/Th2 cytokines in the peripheral blood from surgical patients with primary carcinoma of Liver and to observe the effects after operation. Methods Serum levels of IL - 2, IFN - γ, IL - 4, IL - 10 were detected in 24 patients and 24 healthy subjects by using ELISA, and the mRNA expression of Thl related cytokines （IL- 2,IFN-γ） and Th2 related cytokines （IL- 4, IL -10） were detected by PC R. Results The serum levels and mRNA expression of Th1 cell from primary carcinoma of Liver patients was obviously lower than that in the healthy control （ P 〈 0.05）, but serum levels and mRNA expression of Th2 cell from primary carcinoma of Liver patients was obviously higher than that in healthy control （ P 〈 0.05）. Conclusion It can be discovered that the concentration or the mRNA expression of Thl related cytokines is inhibited with a concomitant predominant state of Th2 related cytokines in the peripheral blood from patients in the primary carcinoma of liver.
出处
《宁夏医学杂志》
CAS
2007年第7期581-583,共3页
Ningxia Medical Journal
基金
福建省自然基金资助项目(编号:C0510016)
