摘要
目的了解骨髓增生异常综合征(MDS)PTEN 基因表达和 Akt 磷酸化(p-Akt)水平之间的关系,探讨 MDS 演进、高风险转归为急性髓系白血病的机制。方法采用 RT-PCR 检测白血病细胞株 K562、Jurkat,65例 MDS 患者骨髓细胞 PTEN mRNA 表达情况。同时用流式细胞术检测 Jurkat、HL-60细胞和30例 MDS 患者骨髓细胞 p-Akt 水平。并以21份正常人骨髓标本为健康对照。结果①PTEN 基因阳性对照 K562细胞呈阳性表达,阴性对照 Jurkat 细胞无表达。65例 MDS 患者中有27例表达阳性,阳性率41.5%,与健康对照组(85.7%)比较差异有统计学意义(P<0.01)。RAEB(39.1%)、RAEB-t(27.8%)和 MDS-AML(12.5%)PTEN 阳性率均低于 RA/RARS(75.0%)组,各亚型组之间比较差异有统计学意义(P<0.01)。②阳性对照 Jurkat 细胞高表达 p-Akt(86.9%),阴性对照 HL-60细胞 p-Akt 阴性。30例 MDS 患者 p-Akt 水平升高(1.35%～58.23%),与健康组(0.54%～2.34%)比较差异有统计学意义(P<0.01),且随着原始细胞比例增高 p-Akt 水平升高。MDS 患者PTEN 基因表达缺失率与 p-Akt 阳性率呈显著正相关(r=0.93,P<0.01)。结论 MDS 患者 PTEN 基因表达缺失是导致 p-Akt 高表达的关键因素之一,并且可能加速 MDS 病程进展及转化为急性髓系白血病。
Objective To investigate the relationship between PTEN gene expression and Akt phosphorylation（p-Akt） in myelodysplastic syndrome（MDS） and to explore the progression of MDS and the mechanism of high risk transformation to acute myeloid leukemia, Methods RT-PCR was used to detect the PTEN mRNA expression in leukemia cell lines K562 （as negative control） and Jurkat （as positive control） and 65 MDS and MDS/AML patients. Flow cytometry was used to detect p-Akt in HL-60 and Jurkat cells and 30 MDS patients. Results ①K562 cells present PTEN gene expression while Jurkat cells did not. Of 65 MDS and MDS/AML patients, 27 （41.5%） expressed PTEN mRNA, being significantly lower than that in normal group（ 85.7% ） （P 〈 0.01 ）. ② Jurkat cell showed high expression（ 86.9% ） of p-Akt, while HL-60 cell as negative control did not express. P-Akt levels of 30 MDS patients were increased （ 1. 35% - 58.23% ）, being much higher as compared with that of the normal contrast group （0.54% - 2.34% ） （ P 〈 0, 01 ）. Moreover, with the rate of blast cells increasing, the p-Akt level was rising up, There is a positive correlation （r =0.93, P 〈0.01 ） between the low expression rate of PTEN and the positive rate of p-Akt, Conclusion The loss of PTEN gene expression is one of the important factors of p-Akt high expression in MDS patients, moreover, it may speed up the progress of the MDS or transformation to acute myeloid leukemia,
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2007年第7期470-473,共4页
Chinese Journal of Hematology
