摘要
目的探讨重组人粒细胞／巨噬细胞集落刺激因子（ｒｈＧＭ－ＣＳＦ）对放疗化疗后动物血象等的影响。方法利用家犬和小白鼠分别服用环磷酰胺和６０Ｃｏ照射制造的动物模型，测定注射ｒｈＧＭ－ＣＳＦ后动物白细胞、血小板、红细胞、血红蛋白、网织细胞、脾集落数、骨髓有核细胞、巨噬细胞及病理组织变化等。结果注射１２和２４μｇ／ｋｇ剂量的ｒｈＧＭ－ＣＳＦ显著抑制家犬由环磷酰胺所致白细胞和血小板的减少；注射７５、１５０、３００μｇ／ｋｇ剂量ｒｈＧＭ－ＣＳＦ显著增加６０Ｃｏ照射后小鼠脾细胞集落、外周白细胞、骨髓有核细胞和巨噬细胞。结论ｒｈＧＭ－ＣＳＦ有明显抑制环磷酰胺及６０Ｃｏ照射所致白细胞减少的作用，且与进口升白能药效相似。
Objective To evaluate the efficacy of recombinant human granulocyte macrophage colony stimulating factor(rhGM CSF)in attenuating the myelosuppression associated with chemotherapy and radiotherapy,compared with import of rhGM CSF。Method The dogs were treated with cyclophosphamide and mice were treated with 60 Co(750cGy) and number of WBC,RBC,CFU S etc. and pathology checking were determinated after injecting rhGM CSF.Results 12 and 24 μg/kg rhGM CSF significantly inhibit the decrease of the absolute WBC and platelet count resulted from cyclophosphamide compared to that of vehicle treated dogs.75,150 and 300 μg/kg rhGM CSF injected mice that were treated with 60 Co significantly increase the number of CFU S,WBC,marrow derived hematopoetic stem cell,and rhagiocrine cell of mice.Conclutions rhGM CSF can significantly inhibit the decreasion of WBC of animal treated by cyclophosphamide and radiation.
出处
《现代诊断与治疗》
CAS
1997年第2期79-81,共3页
Modern Diagnosis and Treatment
