摘要
根据保守氨基酸序列FTQQYQ和anti-1/MIWPNV设计苹果自交不亲和基因引物P1:5′-TT-TACGCAGCAATATCAG-3′;P2:5′-ACGTTCGGCCAAATA/CATT-3′,利用PCR-RFLP分析方法,以河北省新引进的苹果品种美国8号、昂林、松本锦、华冠、南方脆幼叶为试验材料,鉴定其自交不亲和基因型分别为S9S27、S1S9、S9S9、S2S9、S2S9。
Five new apple cultivars, USA No. 8, Korin, Matsumoto Nishiki, Huaguan and Nanfangcui, which were introduced into Hebei Province recently, were used as materials in the present study. The primers （ Pi ： 5＇-TTTACGCAGCAATATCAG-3＇ ; P2 ： 5＇-ACGTrCGGCCAAATA/CATT-3＇） were newly designed according to the conserved sequence of amino acid of S-RNase. The self-incompatibility alleles of the cultivars were isolated and identified using PCR - RFLP analysis. Based on the results, the self-incompatibility genotypes of the cuhivars were identified as S9S27, S1S9, S9S9, S2S9 and S2S9, respectively.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第3期747-750,共4页
Acta Horticulturae Sinica
基金
河北农业大学将帅基金资助项目
关键词
苹果
自交不亲和基因型
分离
鉴定
Apple
Self-incompatibility genotype
Isolation
Identification
