摘要
利用荧光原位杂交技术(Fluorescent in-situ hybridization,FISH)特异性地标记了活性污泥中α-Proteobacteria、β-Proteobacteria、γ-Proteobacteria和Comamonassp.4大类群的微生物,借助流式细胞术(Flow cytometry,FCM)检测了这些类群的相对含量.结果表明,上述微生物的相对含量分别为15.09%、20.50%、12.00%和8.37%.紫外线辐照3min后,活性污泥中菌群的相对含量分别变为20.75%、9.10%、81·89%和41·16%,这说明不同菌群对紫外线的敏感度不同.
Bacteria from the α,β and γ subclasses of Proteobacteria and Comamonas sp. in activated sludge were specifically labeled by fluorescent insitu hybridization （FISH） and flow cytometry （FCM） was used to detect the relative amounts of these four microbial communities. The initial relative quantities were 15.09 % , 20.5 % , 12.00% and 8.37%. After exposure to ultraviolet radiation （UV） for 3 minutes, the relative amounts changed to 20.75 % ,9.10 % ,81.89 % and 41.16 % indicating that the four microbial communities present different sensitivities to UV light.
出处
《环境科学学报》
CAS
CSCD
北大核心
2007年第7期1163-1167,共5页
Acta Scientiae Circumstantiae
基金
欧盟合作项目(No.023168)~~
关键词
流式细胞术
紫外线
活性污泥
flow cytometry
ultraviolet ray
activated sludge
