摘要
建立了固相萃取前处理净化技术．高效液相色谱（HPLC）法检测枸杞中阿维菌素B1a残留量的方法。样品用乙腈提取，经C18固相萃取柱净化，采用C18柱（250mm×4．6mm i．d．，5μm）分离，以V（甲醇）：V（水）=85：15为流动相，在245nm下进行检测。阿维菌素B1a在0．20～2．00μg／mL范围呈良好的线性关系（r=0．9994）；该方法检出限（S／N=3）为0．02mg／kg；添加回收率为88．3％～102．5％，相对标准偏差为2．1％～3．8％。
A method was developed for the determination of avermectin B1a residue in lycium barbarum by HPLC with a clean-up procedure by SPE. Lycium Barbarum was extracted with acetonitrile, purified by Cls SPE colmnn. The analysis was performed on a Cas colmnn （250 mm × 4.6 mm i.d., 5 μm） with methanol-water （85：15） as the mobile phase and detected at 245 ran. Avermectin B1a showed good linear relationship （r = 0.9994） in the range of 0.20- 2.00μg/mL, the limit of detection was 0.02 mg/kg （S/N = 3）. The recoveries ranged from 88.3% to 102.5% with the relative standard deviations （RSDs） from 2.1% to 3.8%.
出处
《分析试验室》
CAS
CSCD
北大核心
2007年第7期97-99,共3页
Chinese Journal of Analysis Laboratory
关键词
高效液相色谱法
固相萃取
阿维菌素B1a
残留分析
枸杞
High performance liquid chromatography
Solid-phase extraction
Avermectin B1a
Residue analysis
Lycium barbarum
